The Wound-Healing Effect of a Novel Fibroblasts-Impregnated Hydroxyethylcellulose Gel in a Rat Full-Thickness Burn Model: A Preclinical Study

The Wound-Healing Effect of a Novel Fibroblasts-Impregnated Hydroxyethylcellulose Gel in a Rat Full-Thickness Burn Model: A Preclinical Study

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Background/Objectives: The objective of this study was to assess the efficacy of a cell-containing wound dressing based on fibroblasts in hydroxyethylcellulose (HEC) gel for the local treatment of deep partial-thickness and/or full-thickness skin burns in an animal model.Methods: The rats (male Wistar, n = 100) were subjected to a Kids Bag full-thickness thermal burn (16 cm2).Radical necrectomy was performed one day after the burn.Three days later, the rats were randomly assigned to one of four groups: group 1 (no treatment), group 2 (chloramphenicol and methyluracil ointment, a routine clinical treatment), group 3 (a gel without cells, mock treatment), and group 4 (a dermal fibroblast-impregnated HEC gel).

The treatment lasted for five days.The wound-healing process was evaluated by planimetric, cytologic, histologic, and immunohistochemical methods.Results: The differences in the rate of wound healing and the characteristics of wound cytology were identified.In the group 4, a regenerative type of cytogram was revealed, characterized by a significantly increased number of fibroblastic cells in comparison to samples Gasket Clamp Pliers from non-treated and mock-treated animals.

Biopsy samples of burn wounds from animals in the group 4l demonstrated the presence of mature granulation tissue and a large number of microvessels.The repair process was stimulated, as evidenced by the increased thickness of newly formed granulation tissue and epidermis in the wound zone, elevated cellularity, and enhanced re-epithelialization activity.The number of Ki-67-positive proliferating cells was significantly higher in group 4 than in the control groups).A small number of non-proliferating donor fibroblasts was observed in the wound area 3 days after the end of treatment.

Conclusions: The cell product is an effective agent for promoting wound healing during the regenerative phase.The experiments demonstrated that a gel populated by dermal fibroblasts can stimulate reparative regeneration processes in deep partial- and full-thickness burn wounds.